Plaque Reduction Technique for Demonstrating Neutralizing Antibodies for Chlamydia

Plaque reduction technique for demonstrating neutralizing antibodies for Chlamydia.

Some members of the genus Chlamydia (Bedsonia or psittacosis-lymphogranuloma venereum-trachoma-inclusion conjunctivitis group of microorganisms) produce plaques in monolayers of mouse fibroblast (L-929) cells. Hyperimmune chicken antisera may be capable of specifically reducing plaque counts. When the test was applied to chlamydiae isolated from avian species, the results indicated that differe...

A plaque reduction test for dengue virus neutralizing antibodies.

The need for an efficient, practical, and accurate laboratory method for measuring dengue virus neutralizing antibody has been recognized by virologists concerned with arboviruses. Methods previously used include measurement of log neutralization indices by serial virus dilution tests with constant serum in suckling mice (1) or tissue culture tubes (2), or measurement of serum titers in sucklin...

Direct immunoperoxidase method for demonstrating Chlamydia psittaci in tissue sections.

Broadly Neutralizing Antibodies for HIV Eradication.

Passive transfer of antibodies has long been considered a potential treatment modality for infectious diseases, including HIV. Early efforts to use antibodies to suppress HIV replication, however, were largely unsuccessful, as the antibodies that were studied neutralized only a relatively narrow spectrum of viral strains and were not very potent. Recent advances have led to the discovery of a l...

Neutralizing antibodies for orthobunyaviruses in Pantanal, Brazil

The Pantanal is a hotspot for arbovirus studies in South America. Various medically important flaviviruses and alphaviruses have been reported in domestic and wild animals in the region. To expand the knowledge of local arbovirus circulation, a serosurvey for 14 Brazilian orthobunyaviruses was conducted with equines, sheep and free-ranging caimans. Sera were tested for specific viral antibodies...